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mng sequence  (Addgene inc)


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    Structured Review

    Addgene inc mng sequence
    Mng Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mneongreen+mng+sequence/pAAV-hSyn1-mNeonGreen+(Plasmid+%2399135)/pm41094146-633-39-42
    Average 93 stars, based on 4 article reviews
    mng sequence - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: DNA Hanger: Surface Free Single-Molecule Blotting Platform
    Article Snippet: The pQE31 vector expression system was used to construct plasmids for the recombinant protein expression of full-length human PABPC1(PABP) ( ). .. The mNeonGreen (mNG) sequence was amplified by PCR from a plasmid (addgene, #98877), and the PCR product was inserted at the N-terminus of the pQE31 expression vector. ..

    Amplification:

    Article Title: DNA Hanger: Surface Free Single-Molecule Blotting Platform
    Article Snippet: The pQE31 vector expression system was used to construct plasmids for the recombinant protein expression of full-length human PABPC1(PABP) ( ). .. The mNeonGreen (mNG) sequence was amplified by PCR from a plasmid (addgene, #98877), and the PCR product was inserted at the N-terminus of the pQE31 expression vector. ..

    Polymerase Chain Reaction:

    Article Title: DNA Hanger: Surface Free Single-Molecule Blotting Platform
    Article Snippet: The pQE31 vector expression system was used to construct plasmids for the recombinant protein expression of full-length human PABPC1(PABP) ( ). .. The mNeonGreen (mNG) sequence was amplified by PCR from a plasmid (addgene, #98877), and the PCR product was inserted at the N-terminus of the pQE31 expression vector. ..

    Plasmid Preparation:

    Article Title: DNA Hanger: Surface Free Single-Molecule Blotting Platform
    Article Snippet: The pQE31 vector expression system was used to construct plasmids for the recombinant protein expression of full-length human PABPC1(PABP) ( ). .. The mNeonGreen (mNG) sequence was amplified by PCR from a plasmid (addgene, #98877), and the PCR product was inserted at the N-terminus of the pQE31 expression vector. ..

    Expressing:

    Article Title: DNA Hanger: Surface Free Single-Molecule Blotting Platform
    Article Snippet: The pQE31 vector expression system was used to construct plasmids for the recombinant protein expression of full-length human PABPC1(PABP) ( ). .. The mNeonGreen (mNG) sequence was amplified by PCR from a plasmid (addgene, #98877), and the PCR product was inserted at the N-terminus of the pQE31 expression vector. ..



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    (A) Fluorescence microscopy analysis. <t>mNG</t> THT1 and mNG THT2 localise to the cell surface in bloodstream and insect stage T . brucei , respectively, as shown by direct <t>mNeonGreen</t> (mNG) fluorescence. (B) Differential RNA-seq expression analysis of THT1 and THT2 genes in BSF and PCF showing developmental control. Total number of reads at the THT1/THT2 locus, applying an uniqueness filter of MapQ>1, are depicted using the Artemis genome browser ; raw transcriptomic data was derived from Hutchinson et al . (C) Western blot analyses indicating stage specific expression of in situ tagged mNG THT1 and mNG THT2 in BSF (left) and PCF and cells differentiated in DTM medium (right). WT, wild-type; EF1α serves as a loading control. (D) Differentiation in DTM medium of mNG THT expressing cells ( mNG THT2 cells shown) was validated by immunofluorescence microscopy with stage specific surface markers, VSG-2 for BSF and EP procyclin for PCF. In A and D, DNA was counter stained with DAPI; Scale bars 5 μm.
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    mng sequence  (Addgene inc)
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    (A) Fluorescence microscopy analysis. <t>mNG</t> THT1 and mNG THT2 localise to the cell surface in bloodstream and insect stage T . brucei , respectively, as shown by direct <t>mNeonGreen</t> (mNG) fluorescence. (B) Differential RNA-seq expression analysis of THT1 and THT2 genes in BSF and PCF showing developmental control. Total number of reads at the THT1/THT2 locus, applying an uniqueness filter of MapQ>1, are depicted using the Artemis genome browser ; raw transcriptomic data was derived from Hutchinson et al . (C) Western blot analyses indicating stage specific expression of in situ tagged mNG THT1 and mNG THT2 in BSF (left) and PCF and cells differentiated in DTM medium (right). WT, wild-type; EF1α serves as a loading control. (D) Differentiation in DTM medium of mNG THT expressing cells ( mNG THT2 cells shown) was validated by immunofluorescence microscopy with stage specific surface markers, VSG-2 for BSF and EP procyclin for PCF. In A and D, DNA was counter stained with DAPI; Scale bars 5 μm.
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    (A) Fluorescence microscopy analysis. <t>mNG</t> THT1 and mNG THT2 localise to the cell surface in bloodstream and insect stage T . brucei , respectively, as shown by direct <t>mNeonGreen</t> (mNG) fluorescence. (B) Differential RNA-seq expression analysis of THT1 and THT2 genes in BSF and PCF showing developmental control. Total number of reads at the THT1/THT2 locus, applying an uniqueness filter of MapQ>1, are depicted using the Artemis genome browser ; raw transcriptomic data was derived from Hutchinson et al . (C) Western blot analyses indicating stage specific expression of in situ tagged mNG THT1 and mNG THT2 in BSF (left) and PCF and cells differentiated in DTM medium (right). WT, wild-type; EF1α serves as a loading control. (D) Differentiation in DTM medium of mNG THT expressing cells ( mNG THT2 cells shown) was validated by immunofluorescence microscopy with stage specific surface markers, VSG-2 for BSF and EP procyclin for PCF. In A and D, DNA was counter stained with DAPI; Scale bars 5 μm.
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    mneongreen (mng) sequence  (Allele Biotechnology)
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    (A) Fluorescence microscopy analysis. <t>mNG</t> THT1 and mNG THT2 localise to the cell surface in bloodstream and insect stage T . brucei , respectively, as shown by direct <t>mNeonGreen</t> (mNG) fluorescence. (B) Differential RNA-seq expression analysis of THT1 and THT2 genes in BSF and PCF showing developmental control. Total number of reads at the THT1/THT2 locus, applying an uniqueness filter of MapQ>1, are depicted using the Artemis genome browser ; raw transcriptomic data was derived from Hutchinson et al . (C) Western blot analyses indicating stage specific expression of in situ tagged mNG THT1 and mNG THT2 in BSF (left) and PCF and cells differentiated in DTM medium (right). WT, wild-type; EF1α serves as a loading control. (D) Differentiation in DTM medium of mNG THT expressing cells ( mNG THT2 cells shown) was validated by immunofluorescence microscopy with stage specific surface markers, VSG-2 for BSF and EP procyclin for PCF. In A and D, DNA was counter stained with DAPI; Scale bars 5 μm.
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    Image Search Results


    (A) Fluorescence microscopy analysis. mNG THT1 and mNG THT2 localise to the cell surface in bloodstream and insect stage T . brucei , respectively, as shown by direct mNeonGreen (mNG) fluorescence. (B) Differential RNA-seq expression analysis of THT1 and THT2 genes in BSF and PCF showing developmental control. Total number of reads at the THT1/THT2 locus, applying an uniqueness filter of MapQ>1, are depicted using the Artemis genome browser ; raw transcriptomic data was derived from Hutchinson et al . (C) Western blot analyses indicating stage specific expression of in situ tagged mNG THT1 and mNG THT2 in BSF (left) and PCF and cells differentiated in DTM medium (right). WT, wild-type; EF1α serves as a loading control. (D) Differentiation in DTM medium of mNG THT expressing cells ( mNG THT2 cells shown) was validated by immunofluorescence microscopy with stage specific surface markers, VSG-2 for BSF and EP procyclin for PCF. In A and D, DNA was counter stained with DAPI; Scale bars 5 μm.

    Journal: PLoS Pathogens

    Article Title: Gluconeogenesis using glycerol as a substrate in bloodstream-form Trypanosoma brucei

    doi: 10.1371/journal.ppat.1007475

    Figure Lengend Snippet: (A) Fluorescence microscopy analysis. mNG THT1 and mNG THT2 localise to the cell surface in bloodstream and insect stage T . brucei , respectively, as shown by direct mNeonGreen (mNG) fluorescence. (B) Differential RNA-seq expression analysis of THT1 and THT2 genes in BSF and PCF showing developmental control. Total number of reads at the THT1/THT2 locus, applying an uniqueness filter of MapQ>1, are depicted using the Artemis genome browser ; raw transcriptomic data was derived from Hutchinson et al . (C) Western blot analyses indicating stage specific expression of in situ tagged mNG THT1 and mNG THT2 in BSF (left) and PCF and cells differentiated in DTM medium (right). WT, wild-type; EF1α serves as a loading control. (D) Differentiation in DTM medium of mNG THT expressing cells ( mNG THT2 cells shown) was validated by immunofluorescence microscopy with stage specific surface markers, VSG-2 for BSF and EP procyclin for PCF. In A and D, DNA was counter stained with DAPI; Scale bars 5 μm.

    Article Snippet: A codon-optimised mNeonGreen (mNG) sequence was cloned using AvrII and PacI (NEB) restriction sites in the pNAT vector [ ].

    Techniques: Fluorescence, Microscopy, RNA Sequencing Assay, Expressing, Derivative Assay, Western Blot, In Situ, Immunofluorescence, Staining